Publications 2020 - 2025
2025
Sawers, R.G. (2025) Dormancy: Metabolite pools prime the cyanobacterial dormancy-resuscitation switch. Curr Biol. 35: R16-R18. doi: 10.1016/j.cub.2024.11.017
Sawers RG. (2025) How FocA facilitates fermentation and respiration of formate by Escherichia coli. J. Bacteriol. e0050224. doi: 10.1128/jb.00502-24
Adrian, L., R.G. Sawers, and D. Deobald (2025) Organohalide respiration in Dehalococcoides strains represents a novel mode of proton motive force generation. Adv. Microbial Physiol. (in press).
2024
Vanyan, L., M. Kammel, M., R.G. Sawers, and K. Trchounian (2024) Evidence for bidirectional formic acid translocation in vivo via the Escherichia coli formate channel FocA. Arch. Biochem. Biophys. 752: 109877. doi: 10.1016/j.abb.2023.109877
Kammel, M., C. Erdmann, and R.G. Sawers (2024) The formate-hydrogen axis and its impact on the physiology of enterobacterial fermentation. Adv. Microbial Physiol. 84: 51-82. doi: 10.1016/bs.ampbs.2024.02.002
Haase, A., C. Arlt, A. Sinz, and R.G. Sawers (2024) Evidence the Isc iron-sulfur cluster biogenesis machinery is the source of iron for [NiFe]-cofactor biosynthesis in Escherichia coli. Sci. Rep. 14: 3026. doi: 10.1038/s41598-024-53745-2
2023
Haase, A., and R.G. Sawers (2023) A redox-active HybG-HypD scaffold complex is required for optimal ATPase activity during [NiFe]-hydrogenase maturation in Escherichia coli. FEBS Open Bio 13: 341-351. doi: 10.1002/2211-5463.13546
Sawers, R.G. (2023) Elucidating the physiology of a microbial cell: Neidhardt’s holy grail. Microbiology Microbiology 120: 54-59. doi: 10.1111/mmi.15051
Kammel, M., and R.G. Sawers (2023) Cooperation between FocA and pyruvate formate-lyase maintains low intracellular formate levels during fermentative growth of Escherichia coli. Fermentation 9: 382. doi.org/10.3390/fermentation9040382
2022
Kammel, M., C. Pinske, and R.G. Sawers (2022) FocA and its central role in fine-tuning pH homeostasis of enterobacterial formate metabolism. Microbiology 168: 10. doi: 10.1099/mic.0.001253
Kammel, M., and R.G. Sawers (2022) Distinguishing functional from structural roles of conserved pore residues during formate translocation by the FocA anion channel. MicrobiologyOpen 11: e1312. doi: 10.1002/mbo3.1312
Kammel, M., O. Trebbin, and R.G. Sawers (2022) Interplay between the conserved pore residues Thr-91 and His-209 controls formate translocation through the FocA channel. Microbial Physiol. 32: 95-107. doi: 10.1159/000524454
Naumann, C., M. Heisters, W. Brandt, P. Janitza, C. Alfs, N. Tang, A. T. Nienguesso, J. Ziegler, R. Imre, K. Mechtler, Y. Dagdas, W. Hoehenwarter, G. Sawers, M. Quint, and S. Abel. (2022) Bacterial-type ferroxidase tunes iron-dependent phosphate sensing during Arabidopsis root development. Curr. Biol. 32: 2189-2205.e6.
doi: 10.1016/j.cub.2022.04.005
Kammel, M., and R.G. Sawers (2022) The autonomous glycyl radical protein GrcA restores activity to inactive full-length pyruvate formate-lyase in vivo J. Bacteriol. 204: e0007022.
doi: 10.1128/jb.00070-22
Haase, A., and R.G. Sawers (2022) Exchange of a single amino acid residue in the HybG chaperone allows maturation of all H2-activating [NiFe]-hydrogenases in Escherichia coli. Frontiers Microbiol. 13: 872581. doi: 10.3389/fmicb.2022.872581
Kammel, M., and R.G. Sawers (2022) The FocA channel functions to maintain intracellular formate homeostasis during Escherichia coli fermentation. Microbiology 168: 4. doi: 10.1099/mic.0.001168
Sargent, F., and R.G. Sawers (2022) A paean to the ineffable Marjory Stephenson. Microbiology 168: 3. doi: 10.1099/mic.0.001160
Kammel, M., O. Trebbin, C. Pinske, and R.G. Sawers (2022) A single amino acid-exchange converts FocA into a unidirectional efflux channel for formate. Microbiology 168: 001132.
doi: 10.1099/mic.0.001132
Sawers, R.G. (2022) In Memoriam: Professor Armen Trchounian (1956-2020). Int. J. Hydrogen Energy 47: 7499-7503. doi.org/10.1016/j.ijhydene.2022.01.029
2021
Arlt, C., K. Nutschan, A. Haase, C. Ihling, D. Tänzler, A. Sinz, and R.G. Sawers (2021) Native mass spectrometry identifies the HybG chaperone as carrier of the Fe(CN)2CO group during maturation of E. coli [NiFe]-hydrogenase 2. Sci Rep 11: 24362–12
Trchounian, K, H. Gevorgyan, R.G. Sawers, and A. Trchounian (2021) Interdependence of Escherichia coli formate dehydrogenase and hydrogen-producing hydrogenases during mixed carbon sources fermentation at different pHs. Int. J. Hydrogen Energy 46: 5085-5099. doi: org/10.1016/j.ijhydene.2020.11.082
Sawers, R.G. (2021) Setting the stage: Genes controlling mechanosensation and Ca2+-signalling in E. coli. J. Bacteriol. 203: e00595-20. doi: 10.1128/JB.00595-20
Falke, D., M. Fischer, C. Ihling, C. Hammerschmidt, A. Sinz, and R.G. Sawers (2021) Co-purification of nitrate reductase 1 with components of the cytochrome bcc-aa3 oxidase supercomplex from spores of Streptomyces coelicolor A3(2). FEBS Open Bio 11: 652-669. doi: 10.1002/2211-5463.13086
Greiner–Haas, F., M. von Bergen, G. Sawers, U. Lechner, and D. Türkowsky (2021) Changes of the proteome and acetylome during transition into the stationary phase in the organohalide-respiring Dehalococcoides mccartyi strain CBDB1. Microorganisms 9: 365. doi: 10.3390/microorganisms9020365
Kammel, M., D. Hunger, and R.G. Sawers (2021) The soluble cytoplasmic N-terminal domain of the FocA channel gates bidirectional formate translocation. Mol. Microbiol. 115(4): 758-773. doi: 10.1111/mmi.14641
2020
Schlaf, G., Bau, D., Horstmann, N., Sawers, R.G., Altermann, W. (2020). Solid phase-based cross-matching for solid organ transplantation: Currently out-of-stock but urgently required for improved allograft outcome. Histol Histopathol 18217. doi:10.14670/HH-18-217
Karapetyan, L., Pinske, C., Sawers, R.G., Trchounian, A. & Trchounian, K. (2020) Influence of C4 -Dcu transporters on hydrogenase and formate dehydrogenase activities in stationary phase-grown fermenting Escherichia coli. IUBMB Life 72: 1680–1685
Benoit S.L., R.J. Maier, R.G. Sawers, and C. Greening (2020) Molecular hydrogen metabolism: a widespread trait of pathogenic bacteria and protists. Microbiol. Mol. Biol. Rev. 84:100.