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Langner U, Baudisch B, Klösgen RB (2014) Organelle import of proteins with dual targeting properties into mitochondria and chloroplasts takes place by the general import pathways. Plant Signal Behav doi: 10.4161/psb.29301 link   

Baudisch B, Langner U, Garz I, Klösgen RB (2014) The exception proves   the rule? Dual targeting of nuclear encoded proteins into  endosymbiotic  organelles. New Phytologist 201(1):80-90 link   

Dittmar J, Schlesier R, Klösgen RB (2014) Tat transport of a Sec passenger leads to both completely translocated as well as membrane-arrested passenger proteins. Biochim Biophys Acta 1843(2):446-53 link   


Hauer RS, Schlesier R, Heilmann K, Dittmar J, Jakob M, Klösgen RB (2013) Enough is enough:TatA demand during Tat-dependent protein transport. Biochim Biophys Acta 1833(5):957-65 link   


Baudisch B and Klösgen RB (2012) Dual targeting of a processing  peptidase into both endosymbiotic organelles mediated by a transport  signal of unusual architecture. Molecular Plant 5(2):494-503 link   

Kerth A, Brehmer T, Meister A, Hanner P, Jakob M, Klösgen RB, Blume A  (2012) Interaction of a Tat substrate and a Tat signal peptide with  thylakoid lipids at the air/water interface. ChemBioChem 13(2):231-9 link   

Price DC, Chan CX, Yoon HS, Yang EC, Qiu H, Weber AP, Schwacke R, Gross J, Blouin NA, Lane C, Reyes-Prieto A, Durnford DG, Neilson JA, Lang BF, Burger G, Steiner JM, Löffelhardt W, Meuser JE, Posewitz MC, Ball S, Arias MC, Henrissat B, Coutinho PM, Rensing SA, Symeonidi A, Doddapaneni H, Green BR, Rajah VD, Boore J, Bhattacharya D (2012) Cyanophora paradoxa genome elucidates origin of photosynthesis in algae and plants. Science 17;335(6070):843-7 link   

Schattat MH, Klösgen RB, Mathur J (2012) New insights on stromules:  Stroma filled tubules extended by independent plastids. Plant Signal  Behav 7:9,1-6 link   


Rödiger A, Baudisch B, Langner U, Klösgen RB (2011) Dual targeting of a mitochondrial protein: The case study of cytochrome c1. Mol Plant. 4(4): 679-87 link   

Schattat M, Barton K, Baudisch B, Klösgen RB, Mathur J (2011) Plastid stromule branching coincides with contiguous ER dynamics. Plant Physiol 155(4): 1667-77 link   

Ladig  R, Sommer MS, Hahn A, Leisegang MS, Papasotiriou DG, Karas M,  Zickermann V, Gutensohn M, Brandt U, Klösgen RB, Schleiff E (2011) A high-definition native polyacrylamide gel electrophoresis system for the analysis of membrane complexes. Plant J 67(1): 181-194 link   

Cho  K, Han Y, Woo JC, Baudisch B, Klösgen RB, Oh S, Han J, Han O (2011)  Cellular localization of dual positional specific maize lipoxygenase-1 in transgenic rice and calcium-mediated membrane association. Plant Sci 181(3): 242-8 link   

Schattat MH, Klösgen RB (2011) Induction of stromule formation by extracellular sucrose and glucose in epidermal leaf tissue of Arabidopsis thaliana. BMC Plant Biol 11: 115 link   


Rödiger A, Baudisch B, Klösgen RB (2010) Simultaneous isolation of  intact mitochondria and chloroplasts from a single pulping of plant  tissue. J Plant Physiol 167(8): 620-624 link   

Fan EG, Jakob  M, Klösgen RB (2010) One signal is enough: Stepwise transport of two  distinct passenger proteins by the Tat pathway across the thylakoid  membrane. Biochem Biophys Res Commun 398(3): 438-443 link   

Schlesier  R, Klösgen RB (2010) Twin arginine translocation (Tat)-dependent  protein transport: the  passenger protein participates in the initial  membrane binding step. Biol Chem 391(12): 1411-1417 link   


Jakob M, Kaiser S, Gutensohn M, Hanner P and Klösgen RB (2009) Tat subunit stoichiometry in Arabidopsis thaliana challenges the proposed function of TatA as the translocation pore. Biochim Biophys Acta - Mol Cell Res 1793(2): 388-94 link   

Schattat MH and Klösgen RB (2009) Improvement of plant cell microscope images by use of 'Depth of Field' - extending software. Endocytobiosis and Cell Research 19: 11-19 link   

Staiger C, Hinneburg A, Klösgen RB (2009) Diversity in Degrees of Freedom of Mitochondrial Transit Peptides. Mol Biol Evol 26(8): 1773-80 link   


Frielingsdorf, S., Jakob, M.  and Klösgen, R.B. (2008) A stromal pool of TatA promotes Tat-dependent protein transport across the thylakoid membrane. J Biol Chem PDF   


Gould, S.B., Fan, E., Hempel, F., Maier, U.G. and Klösgen, R.B. (2007) Translocation of a phycoerythrin alpha subunit across five biological membranes. J Biol Chem, 282, 30295-30302. PDF   

Frielingsdorf, S. and Klösgen, R.B. (2007) Prerequisites for terminal processing of thylakoidal Tat substrates. J Biol Chem, 282, 24455-24462. PDF   


Stumpe, M., Göbel, C., Demchenko, K., Hoffmann, M., Klösgen, R.B., Pawlowski, K. and Feussner, I. (2006) Identification of an allene oxide synthase (CYP74C) that leads to formation of alpha-ketols from 9-hydroperoxides of linoleic and linolenic acid in below-ground organs of potato. Plant J, 47, 883-896. PDF   

Gutensohn, M., Fan, E., Frielingsdorf, S., Hanner, P., Hou, B., Hust, B. and Klösgen, R.B. (2006) Toc, Tic, Tat et al.: structure and function of protein transport machineries in chloroplasts. J Plant Physiol, 163, 333-347. PDF   

Hou, B., Frielingsdorf, S. and Klösgen, R.B. (2006) Unassisted membrane insertion as the initial step in DeltapH/Tat-dependent protein transport. J Mol Biol, 355, 957-967. PDF   

Hust, B. and Gutensohn, M. (2006) Deletion of core components of the plastid protein import machinery causes differential arrest of embryo development in Arabidopsis thaliana. Plant Biol (Stuttg), 8, 18-30. PDF   


Krause, K., Kilbienski, I., Mulisch, M., Rödiger, A., Schäfer, A. and Krupinska, K. (2005) DNA-binding proteins of the Whirly family in Arabidopsis thaliana are targeted to the organelles. FEBS Lett, 579, 3707-3712. PDF   

Müller, M. and Klösgen, R.B. (2005) The Tat pathway in bacteria and chloroplasts (review). Mol Membr Biol, 22, 113-121. PDF   

Zhbanko, M., Zinchenko, V., Gutensohn, M., Schierhorn, A. and Klösgen, R.B. (2005) Inactivation of a predicted leader peptidase prevents photoautotrophic growth of Synechocystis sp. strain PCC 6803. J Bacteriol, 187, 3071-3078. PDF   

Voigt, A., Jakob, M., Klösgen, R.B. and Gutensohn, M. (2005) At least two Toc34 protein import receptors with different specificities are also present in spinach chloroplasts. FEBS Lett, 579, 1343-1349. PDF   

Steiner, J.M., Berghöfer, J., Yusa, F., Pompe, J.A., Klösgen, R.B. and Löffelhardt, W. (2005) Conservative sorting in a primitive plastid. The cyanelle of Cyanophora paradoxa. FEBS J, 272, 987-998. PDF   

Giese, J.O., Herbers, K., Hoffmann, M., Klösgen, R.B. and Sonnewald, U. (2005) Isolation and functional characterization of a novel plastidic hexokinase from Nicotiana tabacum. FEBS Lett, 579, 827-831. PDF   


Klösgen, R.B., Molik, S., Frielingsdorf, S., Gutensohn, M., Jakob, M., Marques, J.P. and Hou, B. (2004) Protein transport across the thylakoid membrane. Endocytobiosis Cell Res, 15, 518-526. PDF   

Gutensohn, M., Pahnke, S., Kolukisaoglu, Ü., Schulz, B., Schierhorn, A., Voigt, A., Hust, B., Rollwitz, I., Stöckel, J., Geimer, S., Albrecht, V., Flügge, U.I. and Klösgen, R.B. (2004) Characterization of a T-DNA insertion mutant for the protein import receptor atToc33 from chloroplasts. Mol Genet Genomics, 272, 379-396. PDF   

Schattat, M.H., Klösgen, R.B. and Marques, J.P. (2004) A novel vector for efficient gene silencing in plants. Plant Molecular Biology Reporter, 22, 145-153. PDF   

Marques, J.P., Schattat, M.H., Hause, G., Dudeck, I. and Klösgen, R.B. (2004) In vivo transport of folded EGFP by the DeltapH/TAT-dependent pathway in chloroplasts of Arabidopsis thaliana. J Exp Bot, 55, 1697-1706. PDF   


Marques, J.P., Dudeck, I. and Klösgen, R.B. (2003) Targeting of EGFP chimeras within chloroplasts. Mol Genet Genomics, 269, 381-387. PDF   


Schleiff, E. and Klösgen, R.B. (2001) Without a little help from `my` friends: direct insertion of proteins into chloroplast membranes? Biochim Biophys Acta, 1541, 22-33. PDF   

Molik, S., Karnauchov, I., Weidlich, C., Herrmann, R.G. and Klösgen, R.B. (2001) The Rieske Fe/S protein of the cytochrome b6/f complex in chloroplasts: missing link in the evolution of protein transport pathways in chloroplasts? J Biol Chem, 276, 42761-42766. PDF   


Berghöfer, J. and Klösgen, R.B. (1999) Two distinct translocation intermediates can be distinguished during protein transport by the TAT (Deltaph) pathway across the thylakoid membrane. FEBS Lett. 460, 328-332. PDF   

Halbig, D., Hou, B., Freudl, R., Sprenger, G.A., and Klösgen, R.B. (1999) Bacterial proteins carrying twin-R signal peptides are specifically targeted by the delta-pH-dependent transport machinery of the thylakoid membrane system. FEBS Lett. 447, 95-98. PDF   

Michl, D., Karnauchov, I., Berghöfer, J., Herrmann, R.G., and Klösgen, R.B. (1999) Phylogenetic transfer of organelle genes to the nucleus can lead to new mechanisms of protein integration into membranes. Plant J. 17, 31-40.

Wexler, M., Bogsch, E.G., Klösgen, R.B., Palmer, T., Robinson, C., and Berks, B.C. (1998) Targeting signals for a bacterial Sec-independent export system direct plant thylakoid import by the delta-pH pathway. FEBS Lett. 431, 339-342.

Karnauchov, I., Herrmann, R.G., Pakrasi, H., and Klösgen, R.B. (1997) Transport of CtpA protein from the cyanobacterium Synechocystis 6803 across the thylakoid membrane in chloroplasts. Eur. J. Biochem. 249, 497-504.

Karnauchov, I., Herrmann, R.G., and Klösgen, R.B. (1997) Transmembrane topology of the Rieske Fe/S protein of the cytochrome b6/f-complex from spinach chloroplasts. FEBS Lett. 408, 206-210.

Klösgen, R.B. (1997) Protein transport into and across the thylakoid membrane. J. Photochem. Photobiol. B: Biology 38, 1-9.

Klösgen, R.B., Berghöfer, J., and Karnauchov, I. (1997) Membrane transport of proteins: a multitude of pathways at the thylakoid membrane. In: Eukaryotism and Symbiosis (Schenk, H.E.A., Herrmann, R.G., Jeon, K.W., Müller, N.E., and Schwemmler, W., eds.), Springer Verlag, Heidelberg New York, pp. 206-213.

Berghöfer, J. and Klösgen, R.B. (1996) Isolation and characterization of a cDNA encoding the SecY protein from spinach chloroplasts (accession no. Z54351) (PGR96-090). Plant Physiol. 112, 863.

Robinson, D., Karnauchov, I., Herrmann, R.G., Klösgen, R.B., and Robinson, C. (1996) Protease-sensitive thylakoidal import machinery for the Sec-, delta-pH- and signal recognition particle-dependent protein targeting pathways, but not for CFo-II integration. Plant J. 10, 149-155.

Herrmann, R.G., Karnauchov, I., Dörfel, P., Altschmied, L., Pakrasi, H., and Klösgen, R.B. (1995) Unexpected diversity of routing processes for nuclear-encoded thylakoid proteins and its phylogenetic impact. In: Photosynthesis: from Light to Biosphere, Vol. III, (Mathis, P., ed.), Kluwer Acad. Publ., Dordrecht London Boston, pp. 713-718.

Reichert, J., Altschmied, L., Klösgen, R.B., Herrmann, R.G., and Haehnel, W. (1995) Interaction of spinach plastocyanin with photosystem I. In: Photosynthesis: from Light to Biosphere, Vol. II, (Mathis, P., ed.), Kluwer Acad. Publ., Dordrecht London Boston, pp. 693-696.

Mant, A., Schmidt, I., Herrmann, R.G., Robinson, C., and Klösgen, R.B. (1995) Sec-dependent thylakoid protein translocation: delta-pH-requirement is dictated by passenger protein and ATP concentration. J. Biol. Chem. 270, 23275-23281.

Brink, S., Fischer, K., Klösgen, R.B., and Flügge, U.-I. (1995) Sorting of nuclear-encoded chloroplast membrane proteins to the envelope and the thylakoid membrane. J. Biol. Chem. 270, 20808-20815.

Berghöfer, J., Karnauchov, I., Herrmann, R.G., and Klösgen, R.B. (1995) Isolation and characterization of a cDNA encoding the SecA protein from spinach chloroplasts: evidence for azide-resistance of Sec-dependent protein translocation across thylakoid membranes of spinach. J. Biol. Chem. 270, 18341-18346.

Chaddock, A.M., Mant, A., Karnauchov, I., Brink, S., Herrmann, R.G., Klösgen, R.B., and Robinson, C. (1995) A new type of signal peptide: central role of a twin-arginine motif in transfer signals for the pH-dependent thylakoidal protein translocase. EMBO J. 14, 2715-2722.

Karnauchov, I., Cai, D., Schmidt, I., Herrmann, R.G., and Klösgen, R.B. (1994) The thylakoid translocation of subunit 3 of photosystem I, the psaF gene product, depends on a bipartite transit peptide and proceeds along an azide-sensitive pathway. J. Biol. Chem. 269, 32871-32878.

Robinson, C. and Klösgen, R. B. (1994) Targeting of proteins into and across the thylakoid membrane - a multitude of mechanisms. Plant Mol. Biol. 26, 15-24.

Herrmann, R.G., Oelmüller, R., Bolle, C., Michl, D., Klösgen, R.B., and Steinmüller, K. (1994) Biogenesis of organelle structure: gene expression and protein sorting. In: Proceedings in Plant Biotechnology (Cho, M.J., ed.), Gyaongsong Natl. Univ. Press, pp. 13-30.

Bolle, C., Sopory, S., Lübberstedt, T., Klösgen, R.B., Herrmann, R.G., and Oelmüller, R. (1994) The role of plastids in the expression of nuclear genes for thylakoid proteins studied with chimeric ß-glucuronidase gene fusions. Plant Physiol. 105, 1355-1364.

Bassham, D.C., Creighton, A.M., Karnauchov, I., Herrmann, R.G., Klösgen, R.B., and Robinson, C. (1994) Mutations at the stromal processing peptidase cleavage site of a thylakoid lumen protein precursor affect the rate of processing but not the fidelity. J. Biol. Chem. 269, 16062-16066.

Michl, D., Robinson, C., Shackleton, J.B., Herrmann, R.G., and Klösgen, R.B. (1994) Targeting of proteins to the thylakoids by bipartite presequences: CFoII is imported by a novel, third pathway. EMBO J. 13, 1310-1317.

Haehnel, W., Jansen, T., Gause, K., Klösgen, R.B., Stahl, B., Michl, D., Huvermann, B., Karas, M., and Herrmann, R.G. (1994) Electron transfer from plastocyanin to photosystem I. EMBO J. 13, 1028-1038.

Robinson, C., Cai, D., Hulford, A., Brock, I.A., Michl, D., Hazell, L., Schmidt, I., Herrmann, R.G., and Klösgen, R.B. (1994) The presequence of a chimeric construct dictates which of two mechanisms are utilised for translocation across the thylakoid membrane: evidence for the existence of two distinct translocation systems. EMBO J. 13, 279-285.

Brock, I. W., Hazell, L., Michl, D., Skovgaard Nielsen, V., Lindberg Møller, B., Herrmann, R.G., Klösgen, R.B., and Robinson, C. (1993). Precursors of one integral and five lumenal thylakoid proteins are imported by isolated pea and barley thylakoids: optimisation of in vitro assays. Plant Mol. Biol. 23, 717-725.

Robinson, C., Klösgen, R.B., Herrmann, R.G., and Shackleton, J.B. (1993) Protein translocation across the thylakoid membrane - a tale of two mechanisms. FEBS Lett. 325, 67-69.

Clausmeyer, S., Klösgen, R.B., and Herrmann, R.G. (1993) Protein import into chloroplasts: the hydrophilic lumenal proteins exhibit unexpected import and sorting specificities in spite of structurally conserved transit peptides. J. Biol. Chem. 268, 13869-13876.

Cai, D., Herrmann, R.G., and Klösgen, R.B. (1993) The 20 kDa apoprotein of the CP24 complex of photosystem II: an alternative model to study import and intra-organellar routing of nuclear-encoded thylakoid proteins. Plant J. 3, 383-392.