Publications 1990 - 1999
1999
Prudencio, M., R. R. Eady and G. Sawers (1999) The blue copper-containing nitrite reductase from Alcaligenes xylosoxidans: cloning of the nirA gene and characterization of the recombinant enzyme. J. Bacteriol. 181: 2323-2329.
Sawers, G. (1999) Biochemistry, physiology and molecular biology of glycyl radical enzymes. FEMS Microbiol. Rev. 22: 543-551.
Hensel, M., A. P. Hinsley, T. Nikolaus, G. Sawers and B. C. Berks (1999) The genetic basis of tetrathionate respiration in Salmonella typhimurium. Mol. Microbiol. 32: 275-288.
Sawers, G. (1999) The aerobic/ anaerobic interface. Current Opin. Microbiol. 2: 181-187.
Richard, D., G. Sawers, F. Sargent and D. H. Boxer (1999) Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology 145: 2903-2912.
Sawers, G. (1999) Glycyl radical enzymes and anaerobic metabolism. John Innes Centre and Sainsbury Laboratory Annual Report 1998/ 1999, p72.
1998
Heßlinger, C., S.A. Fairhurst and G. Sawers (1998) Novel keto acid formate-lyase and propionate kinase activities are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate. Mol. Microbiol. 27: 477-492.
Sawers, G. and G. Watson (1998) A glycyl radical solution: oxygen-dependent interconversion of pyruvate formate-lyase. Mol. Microbiol. 29: 945-954.
Sawers, G. (1998) The anaerobic degradation of L-serine and L-threonine in enterobacteria: networks of pathways and regulatory signals. Arch. Microbiol. 171: 1-5.
Strohmaier, H., R. Noiges, S. Kotschan, G. Sawers, G. Högenauer, E. Zechner and G. Koraimann (1998) Signal transduction and bacterial conjugation: the response regulator ArcA is required for efficient DNA transfer of the conjugative resistance plasmid R1. J. Mol. Biol. 277: 309-316.
Sawers, G., C. Heßlinger, N. Muller and M. Kaiser (1998) The glycyl radical enzyme TdcE can replace pyruvate formate-lyase in glucose fermentation. J. Bacteriol. 180: 3509-3516.
Heßlinger, C. and G. Sawers (1998) The tdcE gene in Escherichia coli strain W3110 is separated from the rest of the tdc operon by insertion of IS5 elements. DNA Sequence 9: 183-188.
Hill, S., N. Juty, E. Kavanagh, M. Merrick and G. Sawers (1998) The physiology of the cytochrome bd-type oxidase in N2-fixing free-living heterotrophs. John Innes Centre and Sainsbury Laboratory Annual Report 1997/ 1998. pp. 34-35
1997
Kaiser, M. and G. Sawers (1997) Overlapping promoters modulate Fnr- and ArcA-dependent anaerobic transcriptional activation of the focApfl operon in Escherichia coli. Microbiology 143: 775-783.
Sawers, G., M. Kaiser, A. Sirko and M. Freundlich (1997) Transcriptional activation by FNR and CRP: reciprocity of binding-site recognition. Mol. Microbiol. 23: 835-845.
Sawers, G. (1997) Multifactorial regulation of bacterial gene expression: integration of responses to extra- and intracellular stimuli. John Innes Centre and Sainsbury Laboratory Annual Report 1996/ 1997. pp. 25-26
Wu, G., S. Hill, M.J.S. Kelly, G. Sawers and R.K. Poole (1997) The cydR gene product, required for cytochrome bd expression in the obligate aerobe Azotobacter vinelandii, is an Fnr-like protein. Microbiology 143: 2197-2207.
1996
Weidner, G. and G. Sawers (1996) Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum. J. Bacteriol. 178: 2440-2444.
Böck, A. and G. Sawers (1996) Fermentation. In Escherichia coli and Salmonella: Molecular and Cellular Biology, 2nd Edition, (Eds. F. C. Neidhardt et al), ASM Press Chapter 18, pp. 262-282.
Sawers, G. and M. Jarsch (1996) Alternative regulation principles for the production of recombinant proteins in Escherichia coli. Appl. Microbiol. Biotechnol. 46: 1-9.
1995
Kaiser, M. and G. Sawers (1995) Nitrate repression of the Escherichia coli pfl operon is mediated by the dual sensors NarQ and NarX and dual regulators NarL and NarP. J. Bacteriol. 177: 3647-3655.
Drapal, N. and G. Sawers (1995) Purification of ArcA and analysis of its specific interaction with the pfl promoter-regulatory region. Mol. Microbiol. 16: 597-607.
Drapal, N. and G. Sawers (1995) Promoter 7 of the Escherichia coli pfl operon is a major determinant in the anaerobic regulation of expression by ArcA. J. Bacteriol. 177: 5338-5341.
Kaiser, M. and G. Sawers (1995) Fnr activates transcription from the P6 promoter of the pfl operon in vitro. Mol. Microbiol. 18: 331-342.
1990-1994
Sawers, G. (1994) The hydrogenases and formate dehydrogenases of Escherichia coli. Antonie van Leeuwenhoek 66: 57-88.
Kaiser, M. and G. Sawers (1994) Pyruvate formate-lyase is not essential for nitrate respiration by Escherichia coli. FEMS Microbiol. Lett. 117: 163-168.
Suppmann, B. and G. Sawers (1994) Isolation and characterisation of hypophosphite-resistant mutants of Escherichia coli: identification of the FOCA protein, encoded by the pfl operon, as a putative formate transporter. Mol. Microbiol. 11: 965-982.
Sawers, G. (1993) Specific transcriptional requirements for positive regulation of the anaerobically inducible pfl operon by ArcA and FNR. Mol. Microbiol. 10: 737-747.
Sirko, A., E. Zehelein, M. Freundlich and G. Sawers (1993) Integration host factor is required for anaerobic pyruvate induction of pfl operon expression in Escherichia coli. J. Bacteriol. 175: 5769-5777.
Sawers, G. and B. Suppmann (1992) Anaerobic induction of pyruvate formate-lyase gene expression is mediated by the ArcA and FNR proteins. J. Bacteriol. 174: 3474-3478.
Böck, A., K. Forchhammer, J. Heider, W. Leinfelder, G. Sawers, B. Veprek and F. Zinoni (1991) Selenocysteine: the 21st amino acid. Mol. Microbiol. 5: 515-520.
Rossmann, R., G. Sawers and A. Böck (1991) Mechanism of regulation of the formate-hydrogenlyase pathway by oxygen, nitrate and pH: definition of the formate regulon. Mol. Microbiol. 5: 2807-2814.
Lutz, S., A. Jacobi, V. Schlensog, R. Böhm, G. Sawers and A. Böck (1991) Molecular characterisation of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli. Mol. Microbiol. 5: 123-135.
Sawers, G., J. Heider and A. Böck (1991) Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme. J. Bacteriol. 173: 4983-4993.
Sawers, R.G. (1991) Identification and molecular characterisation of a transcriptional regulator from Pseudomonas aeruginosa PAO1 exhibiting structural and functional similarity to the FNR protein of Escherichia coli. Mol. Microbiol. 5: 1469-1481.
Knappe, J. and G. Sawers (1990) A radical route to acetyl-CoA: the anaerobically induced pyruvate formate-lyase system of Escherichia coli. FEMS Microbiol. Rev. 75: 383-398.
Böck, A., C. Baron, K. Forchhammer, J. Heider, W. Leinfelder, G. Sawers, B. Veprek, E. Zehelein and F. Zinoni (1990) From nonsense to sense: UGA encodes selenocysteine in formate dehydrogenase and other selenoproteins. In: 41st Moosbach Colloquium, The Molecular Basis of Bacterial Metabolism. Eds. G. Hauska and R. Thauer. Springer-Verlag. pp 61-68.
Sauter, M. and R.G. Sawers (1990) Transcriptional analysis of the gene encoding pyruvate formate-lyase-activating enzyme of Escherichia coli. Mol. Microbiol. 4: 355-363.
Heider, J., K. Forchhammer, G. Sawers and A. Böck (1990) Interspecies compatibility of selenoprotein biosynthesis in Enterobacteriaceae. Arch. Microbiol. 155: 221-228.