Publications 2000 - 2009
2009
Sawers, R.G. (June 2009) Amino Acid Degradation. In: Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: Chichester. DOI: 10.1002/ 9780470015902.a0001388.pub2
2008
Barbieri, S., L.M. Murphy, R.G. Sawers, R.R. Eady and S.S. Hasnain (2008) Modulation of NO-binding to cytochrome c’ by distal and proximal heme pocket residues. J. Biol. Inorg. Chem. 13: 531-540.
2007
Forzi, L. and R.G. Sawers (2007) Maturation of [NiFe]-hydrogenases in Escherichia coli. BioMetals 20: 567-578.
Baker-Austin, C., M. Dopson, M. Wexler, R.G. Sawers and P.L. Bond (2007) Extreme arsenic resistance by the acidophilic archaeon Ferroplasma acidarmanus. Extremophiles 11: 425-434.
Paraskevopoulos, K., M.A. Hough, R.G. Sawers, R.R. Eady and S.S. Hasnain (2007) The structure of Met144Leu copper nitrite reductase from Alcaligenes xylosoxidans provides a first glimpse of a protein-protein complex with azurin II. J. Biol. Inorg. Chem. 12: 789-796.
Burman, J.D., C.E.M. Stevenson, R.G. Sawers and D.M. Lawson (2007) The crystal structure of Escherichia coli TdcF, a member of the highly conserved YjgF/YER057c/UK114 family. BMC Struct. Biol. 7: 30.
McAnulla, C., A. Edwards, M. Sanchez-Contreras, R.G. Sawers and J.A. Downie (2007) Quorum-sensing-regulated transcriptional initiation of plasmid transfer and replication genes in Rhizobium leguminosarum biovar viciae. Microbiology 153: 2074-2082.
Forzi, L., P. Hellwig, R.K. Thauer and R.G. Sawers (2007) The CO and CN ligands to the Fe atom in [NiFe]-hydrogenase-2 of Escherichia coli have different metabolic origins. FEBS Lett 581: 3317-3321.
van Keulen, G., J. Alderson, J. White and R.G. Sawers (2007) The obligate aerobic actinomycete Streptomyces coelicolor A3(2) survives extended periods of anaerobic stress. Env. Microbiol. 9: 3143-3149.
2006
Ellington, M.J.K., W.L. Fosdike, R.G. Sawers, D.J. Richardson and S.J. Ferguson (2006) Regulation of the nap operon encoding the periplasmic nitrate reductase of Paracoccus pantotrophus: delineation of sequences required for redox control. Arch. Microbiol. 184: 298-304.
Reyes-Ramírez, F. and R.G. Sawers (2006) Aerobic activation of the anaerobically inducible Escherichia coli focA-pfl operon transcription by FNR. FEMS Microbiol. Lett. 255: 262-267.
Todd, J.D., G. Sawers, D.A. Rodionov and A.W.B. Johnston (2006) The Rhizobium leguminosarum regulator IrrA affects the transcription of a wide range of genes in response to Fe availability. Mol. Genet. Genom. 275: 564-577.
Sawers, R.G. (2006) Differential turnover of the multiple processed transcripts of the Escherichia coli focA-pflB operon. Microbiology 152: 2197-2205.
Harris, R.L., R.R. Eady, S.S. Hasnain and R.G. Sawers (2006) Coordinate synthesis of azurin I and copper nitrite reductase in Alcaligenes xylosoxidans during denitrification. Arch. Microbiol. 186: 241-249.
Paraskevopoulos, K., S.V. Antonyuk, R.G. Sawers, R.R. Eady and S.S. Hasnain (2006) Insight into catalysis of nitrous oxide reductase from high resolution structures of resting and inhibitor-bound enzyme from Achromobacter cycloclastes. J. Mol. Biol. 362: 55-65.
2005
Sawers, R.G. (2005) Formate and its role in hydrogen production in E. coli. Biochem. Soc. Trans. 33: 42-46.
Harris, R.L., M. Prudencio, S.S. Hasnain, R.R. Eady and R.G. Sawers (2005) Heterologous metalloprotein biosynthesis in Escherichia coli: conditions for the overproduction of functional copper-containing nitrite reductase and azurin from Alcaligenes xylosoxidans. J. Syc. Rad 12: 13-18.
van Keulen, G., J. Alderson, J. White and R.G. Sawers (2005) Nitrate reduction in the actinomycete Streptomyces coelicolor. Biochem. Soc. Trans. 33: 210-212.
Tard, C, X. Liu, S.K. Ibrahim, M. Bruschi, L. De Gioia, S.C. Davies, X. Yang, L.-S, Wang, G. Sawers and C.J. Pickett (2005) Synthesis of the H-cluster framework of iron-only hydrogenase. Nature 433: 610-613.
Sawers, R.G. (2005) Expression of fnr is constrained by an upstream IS5 insertion in certain Escherichia coli K-12 strains. J. Bacteriol. 187: 2609-2617.
Todd, J.D., G. Sawers and A.W.B. Johnston (2005) Proteomic analysis reveals the wide-ranging effects of the novel iron-responsive regulator RirA in Rhizobium leguminosarum bv. viciae. Mol. Genet. Genom. 273: 197-206.
Sawers, R.G. (2005) Transcript analysis of Escherichia coli K-12 insertion element IS5. FEMS Microbiol. Lett. 244: 397-401.
Hough, M.A., M.J. Ellis, S. Antonyuk, R.W. Strange, G. Sawers, R.R. Eady and S.S. Hasnain (2005) High resolution structural studies of mutants provide insights into catalysis and electron transfer processes in copper nitrite reductase. J. Mol. Biol. 350: 300-309.
van Keulen, G., D.A. Hopwood, L. Dijkhuizen and R.G. Sawers (2005) Gas vesicles in actinomycetes: old buoys in novel habitats? Trends Microbiol. 13: 350-354.
Baker-Austin, C., M. Dopson, M. Wexler, R.G. Sawers and P.L. Bond (2005) The molecular basis of extreme copper resistance in the acidophilic archaeon Ferroplasma acidarmanus Microbiology 151: 2551-2561.
Antonyuk, S., R.W. Strange, R.R. Eady, G. Sawers,and S.S. Hasnain (2005) Atomic resolution structures of resting state, substrate- and product-complexed Cu nitrite reductase provide insight into catalytic mechanism. Proc. Natl. Acad. Sci. USA 102: 12041-12046.
Sawers, R.G. (2005) Evidence for novel processing of the anaerobically inducible dicistronic focA-pfl mRNA transcript in Escherichia coli. Mol. Microbiol. 58: 1441-1453.
Diaz-Mireles, E., M. Wexler, J.D. Todd, D. Bellini, A.W.B. Johnston and R.G. Sawers (2005) The manganese-responsive repressor Mur of Rhizobium leguminosarum is a member of the Fur-superfamily that recognises an unusual operator sequence. Microbiology 151: 4071-4078.
2004
Sawers, R.G., J. Alderson and J. White (2004) Streptomycetes and anaerobic stress survival. Microbiol. Austral. 25: 26-27.
Diaz-Mireles, E., M. Wexler, G. Sawers, D. Bellini, J.D. Todd and A.W.B. Johnston (2004) The Fur-like protein Mur of Rhizobium leguminosarum is a Mn2+-responsive transcriptional regulator. Microbiology 150: 1447-1456.
Farver, O., R.R. Eady, G. Sawers, M. Prudencio and I. Pecht (2004) Met144Ala mutation of the copper-containing nitrite reductase from Alcaligenes xylosoxidans reverses the intramolecular electron transfer. FEBS Lett. 561: 173-176.
Sawers, R.G. and M.M. Nakano (2004) Redox(Oxygen)-Dependent Gene Regulation in Facultative Anaerobes. In Strict and Facultative Anaerobes: Medical and Environmental Aspects. eds M.M Nakano, P. Zuber and A.L. Sonnenschein pp. 67-86, Horizon Bioscience.
Punginelli, C., B. Ize, N.R. Stanley, V. Stewart, G. Sawers, B.C. Berks and T. Palmer (2004) mRNA Secondary Structure Modulates Translation of Tat-Dependent Formate Dehydrogenase-N. J. Bacteriol. 186: 6311-6315.
Burman, J.D., R.L. Harris, K.A. Hauton, D.M. Lawson and R.G. Sawers (2004) The iron-sulphur cluster of the Escherichia coli L-serine dehydratase TdcG is essential for catalysis. FEBS Lett. 576: 442-444.
Ellis, M.J, S.V. Antonyuk, R.W. Strange, G. Sawers, R.R. Eady and S.S. Hasnain (2004) Observation of an unprecedented Cu bis-His site: Crystal Structure of the H129V Mutant of Nitrite Reductase. Inorg. Chem. 43: 7591-7593.
Sawers, R.G. and D.P. Clark (2004) Fermentative pyruvate and acetyl CoA metabolism. July 2004, posting date. Chapter 3.5.3. In R. Curtiss III (Editor in Chief), EcoSal--Escherichia coli and Salmonella: Cellular and Molecular Biology. [Online.] http://www.ecosal.org . ASM Press, Washington, D.C.
Sawers, R.G., M. Blokesch and A. Böck (2004) Anaerobic formate and hydrogen metabolism. September 2004, posting date. Chapter 3.5.4. In R. Curtiss III (Editor in Chief), EcoSal--Escherichia coli and Salmonella: Cellular and Molecular Biology. [Online.] http://www.ecosal.org . ASM Press, Washington, D.C.
Yeoman. K.H., A.R.J. Curson, J.D. Todd, G. Sawers and A.W.B. Johnston (2004) Evidence that the Rhizobium regulatory protein RirA binds to cis-acting Iron-Responsive Operators (IROs) at promoters of some Fe-regulated genes. Microbiology 150: 4065-4074.
Richardson, D.J., G. Sawers,and R.J.M. van Spanning (2004) The bioenergetics of periplasmic electron transport systems in bacteria. Encyclopedia of Biological Chemistry (Eds. W.J. Lennarz and M.D. Lane), Elsevier Scientific Press. 3: 231-238.
Barrett, M.L., R.L. Harris, S. Antonyuk, M.A. Hough, M.J. Ellis, G. Sawers, R.R. Eady and S.S. Hasnain (2004) Insights into redox partner interactions and substrate binding in nitrite reductase from Alcaligenes xylosoxidans: Crystal structures of the Trp138His and His313Gln mutants. Biochemistry 43: 16311-16319.
2003
Wexler, M., J.D. Todd, O. Kolade, D. Bellini, A.M. Hemmings, G. Sawers and A.W.B. Johnston (2003) Fur is not the global regulator of iron uptake genes in Rhizobium leguminosarum. Microbiology 149: 1357-1365.
Ellis, M.J., F. E. Dodd, G. Sawers, R.R. Eady and S.S. Hasnain (2003) Atomic resolution structures of native copper nitrite reductase and the active site mutant Asp92Glu. J. Mol. Biol. 328: 429-438.
Burman, J.D., C.E.M. Stevenson, K.A. Hauton, G. Sawers and D. Lawson (2003) Crystallisation and preliminary X-ray analysis of the E. coli hypothetical protein TdcF. Acta Crystallogr. D59: 1076-1078.
Ellington, M.J.K., G. Sawers, H.J. Sears, S. Spiro, D.J. Richardson and S.J. Ferguson (2003) Characterisation of the expression and activity of the periplasmic nitrate reductase of Paracoccus pantotrophus in chemostat cultures. Microbiology 149: 1533-1540.
Reyes-Ramirez, F., P. Dobbin, G. Sawers and D.J. Richardson (2003) Characterisation of the transcriptional regulation of Shewanella frigidimarina iron-induced flavocytochrome c reveals a novel iron-responsive gene regulation system. J. Bacteriol. 185: 4564-4571.
Yeoman, K.H, S. Mitelheiser, G. Sawers and A.W.B. Johnston (2003) The ECF sigma factor RpoI of R. leguminosarum initiates transcription of the vbsGSO and vbsADL siderophore biosynthetic genes in vitro. FEMS Microbiol. Lett. 223: 239-244.
2002
Ellis, M.J., M. Prudêncio, F.E. Dodd, R.W. Strange, G. Sawers, R.R. Eady and S.S Hasnain (2002) Biochemical and crystallographic studies of the Met144Ala, Asp92Asn and His254Phe mutants of nitrite reductase from Alcaligenes xylosoxidans provide insight into the enzyme mechanism. J. Mol. Biol. 316: 51-64.
Carter, R.A., K.H. Yeoman, A. Klein, A.F.H. Hosie, G. Sawers, P.S. Poole and A.W.B. Johnston (2002) dpp genes of Rhizobium leguminosarum specify uptake of gamma-amino-levulinic acid. Mol. Plant-Microbe Interact. 15: 69-74.
Wyborn, N.R., S.L. Messenger, G. Sawers, R.E. Roberts, M.M. Attwood, and J. Green (2002) Expression of the Escherichia coli yfiD gene responds to intracellular pH, and reduces the accumulation of acidic metabolic end-products. Microbiology 148: 1015-1026.
Prudencio, M., G. Sawers, S.A. Fairhurst, F. Yousafzai and R.R. Eady (2002) Alcaligenes xylosoxidans dissimilatory nitrite reductase: alanine substitution of the surface-exposed histidine-139 ligand of the type 1 copper center prevents electron transfer to the catalytic center. Biochemistry 41: 3430-3438.
Richardson, D. and G. Sawers (2002) Structural biology – PMF through the redox loop. Science 295: 1842-1843.
Carter, R.J., P.S. Worsley, G. Sawers, G.L. Challis, M.J. Dilworth, K.C. Carson, J.A. Lawrence, M. Wexler, A.W.B. Johnston and K.H. Yeoman (2002) The vbs genes that direct synthesis of the siderophore vicibactin in Rhizobium leguminosarum: their expression in other genera requires the ECF sigma factor RpoI. Mol. Microbiol. 44: 1153-1166.
Ellington M.J.K., K.K. Bhakoo, G. Sawers, D.J. Richardson and S.J. Ferguson (2002) Hierarchy of carbon source selection in Paracoccus pantotrophus: strict correlation between reduction state of the carbon substrate and aerobic expression of the nap operon. J. Bacteriol. 184: 4767-4774.
Todd, J.D., M. Wexler, G. Sawers, K.H. Yeoman, P.S. Poole and A.W.B. Johnston (2002) RirA, an iron-responsive regulator in the symbiotic bacterium Rhizobium leguminosarum. Microbiology 148: 4059-4071.
2001
Prudencio, M., R.R. Eady and G. Sawers (2001) Characterisation of the Alcaligenes xylosoxidans nitrite reductase type 2 copper centre: mutational analysis of the Asp-92 ligand. Biochem. J. 353: 259-266.
Wu, G., A. J. G. Moir, S. Hill, G. Sawers and R. K. Poole (2001) Biosynthesis of poly-beta-hydroxbutyrate is controlled by CydR (FNR) in the obligate aerobe Azotobacter vinelandii. FEMS. Lett. 194: 215-220.
Jack, R.L., Sargent, F., Berks, B.C., Sawers, G. and Palmer, T. (2001) Constitutive expression of the Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth. J. Bacteriol. 183: 1801-1804.
Sawers, G. (2001) A novel mechanism controls anaerobic and catabolite regulation of the Escherichia coli tdc operon. Mol. Microbiol. 39: 1285-1298.
Ellis M. J., F. E. Dodd, R. W. Strange, M. Prudêncio, G. Sawers, R. R. Eady and S. S. Hasnain (2001) X-ray structure of a blue copper nitrite reductase at high pH and in copper free form at 1.9Å resolution. Acta Crystallogr. D57: 1110-1118.
Wexler, M., K. H. Yeoman, J. B. Stevens, N. G. de Luca, G. Sawers and A. W. B. Johnston (2001) The Rhizobium leguminosarum tonB gene is required for the uptake of siderophore and haem as sources of iron. Mol. Microbiol. 41: 801-816.
Sawers, G. (2001) Amino acid degradation. Encyclopedia of Life Sciences. www.els.net .
2000
Wu, G., H. Cruz-Ramos, S. Hill, J. Green, G. Sawers and R. K. Poole (2000) Regulation of cytochrome bd expression in the obligate aerobe Azotobacter vinelandii by CydR (Fnr): extreme sensitivity to oxygen, recative oxygen species and nitric oxide. J. Biol. Chem. 275: 4679-4686.
Alexeeva, S., B. de Kort, G. Sawers, K. J. Hellingwerf and M. J. Teixeira de Mattos (2000) Effects of limited aeration and of the ArcAB system on intermediary pyruvate catabolism in Escherichia coli. J. Bacteriol. 182: 4934-4940.
Sears, H., G. Sawers, B. C. Berks, S. J. Ferguson and D. Richardson (2000) Control of periplasmic nitrate reductase gene expression in response to oxygen and carbon substrates. Microbiology 146: 2977-2985.
Hill, S. and G. Sawers (2000) Azotobacter. Encyclopedia of Microbiology, 2nd edition Vol. 1 pp. 359-371.