Publications 2010 - 2019
2019
Nutschan, K., R.P. Golbik, and R.G. Sawers (2019) The iron-sulphur-containing HypC-HypD scaffold complex of the [NiFe]-hydrogenase maturation machinery is an ATPase. FEBS Open Bio 9:2072–2079. (doi: 10.1002/2211-5463.12743)
Pinske, C., C. Thomas, K. Nutschan, and R.G. Sawers (2019) Delimiting the function of the C-terminal extension of the Escherichia coli [NiFe]-hydrogenase 2 large-subunit precursor. Frontiers Microbiol. 10: 2223. (doi: 10.3389/fmicb.2019.02223)
Sawers R.G., M. Fischer, D. Falke (2019) Anaerobic nitrate respiration in the aerobe Streptomyces coelicolor A3(2): Helping maintain a proton gradient during dormancy. Environ. Microbiol. Rep. 11(5): 654-650. (doi: 10.1111/1758-2229.12781)
Fischer, M., D. Falke, J. Rönitz, A. Haase, T. Damelang, T. Pawlik, and R.G. Sawers (2019) Hypoxia-induced synthesis of respiratory nitrate reductase 2 of Streptomyces coelicolor A3(2) depends on the histidine kinase OsdK in mycelium but not in spores. Microbiology 165: 905-916. (doi: 10.1099/mic.0.000829)
Bau, D., R.G. Sawers, A. Wahle, W. Altermann, and G. Schlaf (2019) Solid phase-based cross-matching using the Luminex platform for solid organ allo-grafting: rather regression than progress in comparison to the ELISA-based precursor procedure. J. Clin. Cell Immunol. 10: 1000576. (doi: 10.4172/2155-9899. 1000576)
Budhraja, R., C. Ding, P. Walter, S. Wagner, T. Reemtsma, R.G. Sawers, and L. Adrian (2019) The impact of species, respiration type, growth phase and genetic inventory on absolute metal content of intact bacterial cells. Metallomics 11:925-935. (doi: 10.1039/c9mt00009g)
Falke, D., B. Biefel, A. Haase, S. Franke, M. Fischer, and R.G. Sawers (2019) Activity of spore-specific respiratory nitrate reductase 1 of Streptomyces coelicolor A3(2) requires a functional cytochrome bcc-aa3 oxidase supercomplex. J. Bacteriol. 201:e00104-19. (doi: 10.1128/JB.00104-19)
2018
Falke, D., M. Fischer, B. Biefel, C. Ihling, C. Hammerschmidt, K. Reinefeld, A. Haase, Andrea Sinz, and R.G. Sawers (2018) Cytochrome bcc-aa3 oxidase supercomplexes in the aerobic respiratory chain of Streptomyces coelicolor A3(2). J. Mol. Microbiol. Biotechnol. 28:255-268. (doi: 10.1159/000496390)
Dragomirova, N., P. Rothe, S. Schwoch, S. Hartwig, C. Pinske, and R.G. Sawers (2018) Insights into the redox sensitivity of Chloroflexi Hup-hydrogenase derived from studies in Escherichia coli: Merits and pitfalls of heterologous NiFe-hydrogenase synthesis. Front. Microbiol. 9:2837.
Hakobyan, B., C. Pinske, R.G. Sawers, A. Trchounian, and K. Trchounian (2018) PH and a mixed carbon-substrate spectrum influence FocA- and FocB-dependent, formate-driven H2 production in Escherichia coli. FEMS Microbiol Lett. 365(21).
Fischer, M., D. Falke, C. Naujoks, and R.G. Sawers (2018) Cytochrome bd oxidase has an important role in sustaining growth and development of Streptomyces coelicolor A3(2) under oxygen-limiting conditions. J Bacteriol. 200(16):47.
Thomas, C., M. Waclawek, K. Nutschan, C. Pinske, and R.G. Sawers (2018) The extended C-terminal alpha-helix of the HypC chaperone restricts recognition of large subunit precursors by the Hyp-scaffold machinery during [NiFe]-hydrogenase maturation in Escherichia coli. J. Mol. Microbiol. Biotechnol. 28(2): 87-97.
Schubert, T., L. Adrian, R.G. Sawers, and G. Diekert (2018) Organohalide respiratory chains: composition, topology, and key enzymes. FEMS Microbiol. Ecol. 94(4).
Sawers R.G. (2018) o-phthalate derived from plastics’ plasticizers and a bacterium’s solution to its anaerobic degradation. Mol. Microbiol. 108(6):595-600.
2017
Hunger D., M. Röcker, D. Falke, H. Lilie, and R.G. Sawers (2017) The C-terminal six amino acids of the FNT channel FocA are required for formate translocation but not homopentamer integrity. Front Microbiol 8, 3633.
Hartwig S., N. Dragomirova, A. Kublik, D. Türkowsky, M. von Bergen, U. Lechner, L. Adrian, and R.G. Sawers (2017) A H2 -oxidizing, 1,2,3-trichlorobenzene-reducing multienzyme complex isolated from the obligately organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1. Environ Microbiol Rep. doi:10.1111/1758-2229.12560
Trchounian K., R.G. Sawers, and A. Trchounian (2017) Improving biohydrogen productivity by microbial dark- and photo-fermentations: Novel data and future approaches. Renewable and Sustainable Energy Reviews 80, 1201–1216.
Jaroschinsky M., C. Pinske, and R.G. Sawers (2017) Differential effects of isc operon mutations on the biosynthesis and activity of key anaerobic metalloenzymes in Escherichia coli. Microbiology (Reading, Engl) 163:878-890.
Sawers R.G., and C. Pinske (2017) “[NiFe]-Hydrogenase Assembly”, in ‘Encyclopedia of Inorganic and Bioinorganic Chemistry’, edited by Michael K. Johnson and Robert A. Scott. Chichester, UK: John Wiley & Sons, Ltd, pp. 1-11.
Nies D.H., J. Covès, and R.G. Sawers (2017) Cross-talk between nickel and other metals in microbial systems. In Biological Chemistry of Nickel, Chapter 15. Royal Society of Chemistry, (print publication 04/04/2017) DOI:10.1039/9781788010580-00306.
Adamson H., M. Robinson, P.S. Bond, B. Soboh, K. Gillow, A.N. Simonov, D.M. Elton, A.M. Bond, R.G. Sawers, D.J. Gavaghan, and A. Parkin (2017) Analysis of HypD Disulfide Redox Chemistry via Optimization of Fourier Transformed ac Voltammetric Data. Analytical Chemistry 89:1565-1573
2016
Kublik, A, D. Deobald, S. Hartwig, C.L. Schiffmann, A. Andrades, M. von Bergen, R.G. Sawers, and L. Adrian (2016) Identification of a multiprotein reductive dehalogenase complex in Dehalococcoides mccartyi strain CBDB1 suggests a protein-dependent respiratory electron transport chain obviating quinone involvement. Environ. Microbiol. 18: 3044-3056. doi: 10.1111/1462-2920.13200.
Sawers, R.G. (2016) Little red floaters: gas vesicles in an enterobacterium. Environ. Microbiol. 18:1091-1093. doi: 10.1111/1462-2920.13245.
Sawers, R.G., D. Falke, and M. Fischer (2016) Oxygen and nitrate respiration in Streotpmyces coelicolor A3(2). Adv. Microbial Physiol. 68: 1-40. doi: 10.1016/bs.ampbs.2016.02.004.
Falke, D., C. Doberenz, D. Hunger, and R.G. Sawers (2016) The glycyl-radical enzyme 2-ketobutyrate formate-lyase, TdcE, interacts specifically with the formate-translocating FNT-channel protein FocA. Biochem. Biophys. Reports 6: 185–189. doi: 10.1016/j.bbrep.2016.04.005.
Falke, D., M. Fischer, and R.G. Sawers (2016) Phosphate- and oxygen-limitation induce respiratory nitrate reductase 3 synthesis in stationary-phase mycelium of Streptomyces coelicolor A3(2). Microbiology 162: 1689-1697. doi: 10.1099/mic.0.000349.
Pinske, C., and R.G. Sawers (2016) Anaerobic formate and hydrogen metabolism. EcoSal Plus 2016 (posted 4th October 2016); doi:10.1128/ecosalplus.ESP-0011-2016.
Sawers, R.G. (2016) Dormancy: Illuminating How a Microbial Sleeping Beauty Awakens. Curr. Biol. 26, R1139–R1141. doi: 10.1016/j.cub.2016.08.039
2015
Pinske, C., M. Jaroschinsky, S. Linek, C.L. Kelly, F. Sargent, and R.G. Sawers (2015) Physiology and bioenergetics of [NiFe]-hydrogenase 2-catalyzed H2-consuming and H2-producing reactions in Escherichia coli. J. Bacteriol. 197: 296-306. doi: 10.1128/JB.02335-14.
Sawers, R.G. (2015) Of mothballs and old yellow enzymes. Mol Microbiol. 95: 157–161. doi: 10.1111/mmi.12874.
Sawers, R.G. (January 2015) Amino Acid Degradation. In: eLS. John Wiley & Sons, Ltd: Chichester. doi: 10.1002/9780470015902.a0001388.pub34.
Pinske, C., F. Sargent, and R.G. Sawers (2015) SlyD-dependent nickel delivery limits maturation of [NiFe]-hydrogenases in late-stationary phase Escherichia coli cells. Metallomics 7: 683-690. doi: 10.1039/c5mt00019j
Hartwig, S., C. Pinske, and R.G. Sawers (2015) Chromogenic assessment of the three molybdo-selenoprotein formate dehydrogenases in Escherichia coli. Biochem. Biophys. Rep. 1: 62-67. doi:10.1016/j.bbrep.2015.03.006
Stripp, S.T., U. Lindenstrauss, R.G. Sawers, and B. Soboh (2015) Identification of an isothiocyanate on the HypEF complex suggests a route for efficient cyanyl-group channeling during [NiFe]-hydrogenase cofactor generation. PLoS One 10: e0133118. doi: 10.1371/journal.pone.0133118.
Thomas, C., E. Muhr, R.G. Sawers (2015) Coordination of synthesis and assembly of a modular membrane-associated [NiFe]-hydrogenase is determined by cleavage of the C-terminal peptide. J. Bact. 197: 2989-2998. doi:10.1128/JB.00437-15.
2014
Trchounian, A. and R.G. Sawers (2014) Novel insights into the bioenergetics of mixed-acid fermentation: Can hydrogen and proton cycles combine to help maintain a proton motive force? IUBMB Life 66; 1-7. doi: 10.1002/iub.1236.
Pinske, C. and R.G. Sawers (2014) The importance of iron in the biosynthesis and assembly of [NiFe]-hydrogenases. Biomolecular Concepts 5; 55-70. doi: 10.1515/bmc-2014-0001.
Jaroschinsky, M., and R.G. Sawers (2014) Ferredoxin has a pivotal role in the biosynthesis of the hydrogen-oxidizing hydrogenases in Escherichia coli. Int. J. Hydrogen Energy 39: 18533-18542. doi.org/10.1016/j.ijhydene.2014.03.124.
Hunger, D., C. Doberenz, and R.G. Sawers (2014) Identification of key residues in the formate channel FocA that control import and export of formate. Biol. Chem. 395: 813-825. doi: 10.1515/hsz-2014-0154.
Doberenz, C., M. Zorn, D. Falke, D. Nannemann, D. Hunger, L. Beyer, C.H. Ihling, J. Meiler, A. Sinz, and R.G. Sawers (2014) Pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation. J. Mol. Biol. 426: 2827–2839. doi: 10.1016/j.jmb.2014.05.023.
Fischer, M., D. Falke, and R.G. Sawers (2014) Determination of oxygen respiration rates in wetted developmentally arrested spores of Streptomyces species. Bio-protocol 4(12): http://www.bio-protocol.org/wenzhang.aspx?id=1153 .
Fischer, M., D. Falke, and R.G. Sawers (2014) Rapid nitrate reduction assay with intact microbial cells or spores. Bio-protocol 4(12): http://www.bio-protocol.org/wenzhang.aspx?id=1154 .
Stripp, S.T., U. Lindenstrauss, C. Granich, R.G. Sawers, and B. Soboh (2014) The Influence of oxygen on [NiFe]-hydrogenase cofactor biosynthesis and how ligation of carbon monoxide precedes cyanation. PLoS One 9: e107488. doi: 10.1371/journal.pone.0107488.
Fischer, M., D. Falke, T. Pawlik, and R.G. Sawers (2014) Oxygen-dependent control of respiratory nitrate reduction in mycelium of Streptomyces coelicolor A3(2). J. Bacteriol. 196: 4152–4162. doi: 10.1128/JB.02202-14.
Zorn, M., C.H. Ihling, R. Golbik, R.G. Sawers, and A. Sinz (2014) Mapping cell envelope and periplasm protein interactions of Escherichia coli respiratory formate dehydrogenases by chemical cross-linking and mass spectrometry. J. Proteome Res. 13: 5524-5535. doi: 10.1021/pr5004906.
2013
Bräuer, L., M. Schicht, D. Worlitzsch, T. Bensel, R.G. Sawers and F. Paulsen (2013) Staphylococcus aureus and Pseudomonas aeruginosa express and secrete human surfactant proteins. PLoS One 8: e53705. doi: 10.1371/journal.pone.0053705.
Beyer, L., C. Doberenz, D. Falke, D. Hunger, B Suppmann and R.G. Sawers (2013) Coordinating FocA and pyruvate formate-lyase synthesis in Escherichia coli: preferential translocation of formate over other mixed-acid fermentation products. J. Bacteriol. 195: 1428-1435. doi: 10.1128/JB.02166-12.
Trchounian, K., B. Soboh, R.G. Sawers, A. Trchounian (2013) Contribution of hydrogenase 2 to stationary phase H2 production by Escherichia coli during fermentation of glycerol. Cell. Biochem. Biophys. 66: 103-108. doi: 10.1007/s12013-012-9458-7.
Wagner A., L. Segler, S. Kleinsteuber, G. Sawers, H. Smidt and U. Lechner (2013) Regulation of reductive dehalogenase gene transcription in Dehalococcoides mccartyi. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 368: 20120317. doi: 10.1098/rstb.2012.0317.
Zorn, M., C.H. Ihling, R. Golbik, R.G. Sawers and A. Sinz (2013) Selective selC-independent selenocysteine incorporation into formate dehydrogenases. PLoS One 8: e61913. doi: 10.1371/journal.pone.0061913.
Pinske, C., M. Jaroschinsky, and R.G. Sawers (2013) Levels of control exerted by the Isc iron-sulphur cluster system on the biosynthesis of the formate hydrogenlyase complex. Microbiology 159: 1179-1189. doi: 10.1099/mic.0.066142-0.
Stripp, S.T., B. Soboh, U. Lindenstrauss, M. Braussemann, M. Herzberg, D. H. Nies, R.G. Sawers, and J. Heberle (2013) HypD is the scaffold protein for Fe–(CN)2CO cofactor assembly in [NiFe]–hydrogenase maturation. Biochemistry 52: 3289–3296. doi: 10.1021/bi400302v.
Fischer, M. and R.G. Sawers (2013) A universally applicable and rapid method for measuring growth of Streptomyces and other filamentous microorganisms by methylene blue adsorption/desorption. Appl. Environ. Microbiol. 79: 4499-4502. doi: 10.1128/AEM.00778-13.
Soboh, B., S.T. Stripp, C. Bielak, U. Lindenstrauß, M. Braussemann, M. Javaid, M. Hallensleben, C. Granich, M. Herzberg, J. Heberle and R.G. Sawers (2013) The [NiFe]-hydrogenase accessory chaperones HypC and HybG of Escherichia coli are iron- and carbon dioxide-binding proteins. FEBS Lett. 587: 2512–2516. doi: 10.1016/j.febslet.2013.06.055.
Fischer M., D. Falke, and R.G. Sawers (2013) A respiratory nitrate reductase active exclusively in resting spores of the obligate aerobe Streptomyces coelicolor A3(2). Mol. Microbiol. 89: 1259-1273. doi: 10.1111/mmi.12344.
Poladyan, A., K. Trchounian, R.G. Sawers, and A. Trchounian (2013) Hydrogen-oxidizing hydrogenases 1 and 2 of Escherichia coli regulate the onset of hydrogen evolution and ATPase activity, respectively, during glucose fermentation at alkaline pH. FEMS Microbiol. Lett. 348: 143-148. doi: 10.1111/1574-6968.12281.
Sawers R.G. (2013) Nickel in Bacteria and Archaea. Chapter 86 In: Encyclopedia of Metalloproteins. (Kretsinger, R. H., Uversky, V. N. and Permyakov, E. A., Eds.) Springer, Heidelberg. pp. 1490-1496. ISBN 978-1-4614-1532-9.
Soboh, B. and R.G. Sawers (2013) [NiFe]-hydrogenase cofactor assembly. In: Encyclopedia of Inorganic and Bioorganic Chemistry – Metals in Cells, Chapter eibc2154. ISBN: 9781119951438. doi: 10.1002/9781119951438.eibc2154.
2012
Pinske, C. and R.G. Sawers (2012) The A-type carrier protein ErpA is essential for formation of an active formate-nitrate respiratory pathway in Escherichia coli K-12. J. Bacteriol. 194: 346-353. doi: 10.1128/ JB.06024-11
Pinske, C, J.S. McDowall, F. Sargent and R.G. Sawers (2012) Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12. Microbiology 158: 856-868. doi: 10.1099/mic.0.056622-0
Pinske, C, and R.G. Sawers (2012) Delivery of iron-sulfur clusters to the hydrogen-oxidizing [NiFe]-hydrogenases in Escherichia coli requires the A-type carrier proteins ErpA and IscA. PLoS ONE 7: e31755. doi:10.1371/journal.pone.0031755
Trchounian, K., C. Pinske, R.G. Sawers and Trchounian, A. (2012) Characterization of Escherichia coli NiFe-hydrogenase distribution during fermentative growth at different pHs. Cell Biochem. Biophys. 62: 433-440. doi: 10.1007/s12013-011-9325-y
Kirchberg, J., D. Büttner, B. Thiemer and R.G. Sawers (2012) Aconitase B is required for optimal growth of Xanthomonas campestris pv. vesicatoria in pepper plants. PLoS ONE 7: e34941. doi:10.1371/journal.pone.0034941
Fischer, M., C. Schmidt, D. Falke and R.G. Sawers (2012) The terminal reduction reactions of nitrate and sulphate assimilation in Streptomyces coelicolor A3(2): identification of the genes encoding the nitrite and sulphite reductases. Res. Microbiol. 163: 340-348. doi: 10.1016/j.resmic.2012.05.004
Soboh, B., M. Kuhns, M. Braussemann, M. Waclawek, E. Muhr, A.J. Pierik and R.G. Sawers (2012) Evidence for an oxygen-sensitive iron-sulfur cluster in an immature large subunit species of Escherichia coli [NiFe]-hydrogenase 2. Biochem. Biophys. Res. Commun. 424: 158-163. doi: 10.1016/j.bbrc.2012.06.096
Pinske, P., M. Jaroschinsky, F. Sargent, and G. Sawers (2012) Zymographic differentiation of [NiFe]-hydrogenases 1, 2 and 3 of Escherichia coli K-12. BMC Microbiol. 12:134. doi: 10.1186/1471-2180-12-134
Soboh, B., S. Stripp, E. Muhr, C. Granich, M. Braussemann, M. Herzberg, J. Heberle and R.G. Sawers (2012) [NiFe]-hydrogenase maturation: isolation of a HypC-HypD complex carrying diatomic CO and CN- ligands. FEBS Lett. 586: 3882-3887. doi: 10.1016/j.febslet.2012.09.019
Sawers R.G. (2012) Hydrogenase maturation endopeptidase. Chapter 70 In: Handbook of Proteolytic Enzymes. 3rd Edition. (Eds. Rawlings, N. and Salvesen, G.) Academic Press, ISBN: 9780123822192
2011
Pinske, C., S. Krüger, B. Soboh, C. Ihling, M. Kuhns, M. Braussemann, M. Jaroschinsky, C. Sauer, F. Sargent, A. Sinz, and R.G. Sawers (2011) Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulphur cluster-containing small subunit. Arch. Microbiol. 193: 893-903.
Pinske, C., M. Bönn, S. Krüger, U. Lindenstrauß, and R.G. Sawers (2011) Metabolic deficiences revealed in the biotechnologically important model bacterium Escherichia coli BL21(DE3). PLoS ONE 6: e22830.
Soboh B, C. Pinske, M. Kuhns, M. Waclawek, C. Ihling, K. Trchounian, A. Trchounian, A. Sinz and G. Sawers (2011) The respiratory molybdo-selenoprotein formate dehydrogenases of Escherichia coli have hydrogen: benzyl viologen oxidoreductase activity. BMC Microbiol. 11: 173.
Pinske, C. and G. Sawers (2011) Iron restriction induces preferential down-regulation of H2-consuming over H2-evolving reactions during fermentative growth of Escherichia coli. BMC Microbiol. 11: 196.
Petkun, S., R. Shi, Y. Li, A. Asinas, C. Munger, M. Waclawek, B. Soboh, R.G. Sawers and M. Cygler (2011) Structure of hydrogenase maturation protein HypF with reaction intermediates shows two active sites. Structure 19: 1773-1783.
Trchounian, K., C. Pinske, R.G. Sawers and Trchounian, A. (2011) Dependence on the F0F1-ATP synthase for the activities of the hydrogen-oxidizing hydrogenases 1 and 2 during glucose and glycerol fermentation at high and low pH in Escherichia coli. J. Bioenerg. Biomembr. 43: 645-650.
2010
Bekker, M., S. Alexeeva, W. Laan, G. Sawers, J. Teixeira de Mattos, and K. Hellingwerf (2010) The ArcAB two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone pool. J. Bact. 192:746-754.
Falke, D., K. Schulz, C. Doberenz, L. Beyer, H. Lilie, B. Thiemer and R.G. Sawers (2010) Unexpected oligomeric structure of the FocA formate channel of Escherichia coli: A paradigm for the Formate-Nitrite-Transporter family of integral membrane proteins. FEMS Microbiol. Lett. 303: 69-75.
Harris, R.L., S. Barbieri, K. Papraskevopoulos, L.M. Murphy, R.R. Eady, S.S. Hasnain and R.G. Sawers (2010) Characterisation of cycP gene expression in Achromobacter xylosoxidans NCIMB 11015 and high-level heterologous synthesis of cytochrome c' in Escherichia coli. J. Mol. Microbiol. Biotechnol. 18:102–108.