Dr. Barbara Thiemer
Research interests
1. Defense agains oxidative stress in "strict" anaerobic bacteria (T. Wolsch / B. Thiemer, C. Doberenz)
- functions of "new" selenoproteins as revealed by stress parameters and 75Se + 2D-PAGE
- characterization of the selenoperoxiredoxin system of E. acidaminophilum
- identification and characterization of enzymes for detoxification of reactive oxygen species
2. Osmoprotection in anaerobic bacteria (T. Wolsch)
- identification of induced enzymes by 2D-PAGE
- proof of compatible solutes formed
3. Glycine metabolism in E. acidaminophiloum (A. Poehlein)
- biochemical and genetic characterization of glycine decarboxylase
- function of the 11 kDa selenoprotein PrpU
4. Aerobic biotransformation of pyrrole-2-cyrboxylate (T. Wolsch)
- biochemical and genetic characterization of the initiating enzymes:
- flavin-containing monooxygenases of Arthrobacter and Rhodococcus
5. Tetrahydrofuran-Abbau in Pseudonocardia tetrahydrofuranoxydans (B. Thiemer)
Publications
B. Thiemer, J.R. Andreesen, T. Schräder (1999): A covalently bound flavin discovered in the reductase component of a tetrahydrofuran–induced multicomponent monooxygenase. Beitrag zum Tagungsband "Flavins and Flavoproteins – 1999", pp. 879–882. (S. Ghisla, P. Kroneck, P. Macheroux, H. Sund, Eds.). Agency of Scientific Publ., Berlin.
U. Kohlweyer, B. Thiemer, T. Schräder, J.R. Andreesen (2000): Tetrahydrofuran degradation by a newly isolated culture of Pseudonocardia sp. strain K1. FEMS Microbiol. Lett. 186: 301–306.
B. Thiemer, J.R. Andreesen, T. Schräder (2001): The NADH-dependent reductase of a putative multicomponent tetrahydrofuran mono-oxygenase contains a covalently bound FAD. Eur. J. Biochem. 268: 3774-3782.
T. Schräder, B. Thiemer, J.R. Andreesen (2002): A molybdenum-containing dehydrogenase catalyzing an unusual 2-hydroxylation of nicotinic acid. Appl. Microbiol. Biotechnol. 58: 612-617.
B. Thiemer, J.R. Andreesen, T. Schräder (2003): Cloning and characterization of a gene cluster involved in tetrahydrofuran degradation in Pseudonocardia sp. strain K1. Arch. Microbiol. 179: 266-277.
P. Kämpfer, U. Kohlweyer, B. Thiemer, J. R. Andressen (2006): Pseudonocardia tetrahydrofuranoxydans sp. nov. Int J Syst Evol Microbiol. 56:1535-8.